By makeproject

D.I.Y. P.C.R. NOTES, APPENDUM, SHOOTING THE BREEZE – WHATEVER

(This post will be updated from time to time, as improvements arise with regards to the MAKE magazine thermal cycler project. For more info, go here) – – – Date: August 25th We repeated the experiment with the 2C adjustment, but obtained similarily non-optimal data as before. This led to recalibrating of our temperture sensor at 0C and monitoring to see if the temperture cycles waiver at the latter cycles (i.e after the first 10 cycles), which was not the case. At that point, we decided to calibrate our temperature sensor in real time with the eppendorf cycler to see…